Cleaver Scientific Launch Safe Series DNA Electrophoresis Products

29 Apr 2015
Lauren Edwards
Editorial

Product news

The 'Safe' Series of products from Cleaver Scientific have been designed to make DNA electrophoresis procedures safer, more convenient and more economical to run.

It is widely acknowledged that the main hazards of traditional DNA gel electrophoresis lie in the use of Ethidium Bromide stain, a known mutagen and carcinogen, and using UV illumination sources which can cause eye and skin damage. Furthermore, traditional DNA electrophoresis is usually performed without researchers knowing much about what is happening on the gel unit until it is stopped and checked. This blind 'stop and start’ process, where an electrophoresis run is stopped at the time the researcher ‘thinks’ that the samples have separated out enough often makes getting optimised results from traditional DNA gel electrophoresis time-consuming and costly.

Cleaver Scientific's ‘Safe’ Series offers a safer and more user-friendly alternative to traditional methods as it eliminates the need for Ethidium Bromide and UV light, without compromising the results of electrophoresis procedures. This is accomplished combining the merits of 3 innovative products - runSAFE, runDOC and runVIEW®.

runSAFE, is a new and completely safe stain for DNA gel electrophoresis. Cleaver Scientific's runVIEW® is an innovative new system which can be used to maximise the efficiency of DNA recovery from stained gels by minimising the number of steps involved in post-electrophoretic purification. runVIEW® uses a blue LED gel illuminator which is inherently safe. runDOC is a benchtop gel documentation system which sits above the gel tank and enables multiple real-time images of the process to be taken.

Combining Cleaver Scientific's runVIEW®, runSAFE and runDOC system provides a safe, convenient and easy-to-use integrated solution for DNA gel electrophoresis covering each stage of the process. Using runVIEW® and runSafe, researchers can now separate and view DNA bands in real-time. Having complete control of the electrophoresis experiment enables optimised results to be easily and quickly achieved. Further the integrated system enables casting, loading, running, staining, viewing and documentation of DNA and RNA gels in a completely safe environment which requires no additional equipment, space or dark room.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Laboratory SafetyLaboratory safety is essential to the effective operation of all laboratories and is required to minimize the risk of harm during experiments. Guidelines, regulations, and equipment should be available in all laboratories to ensure that the working environment is safe and secure.
Cleaver Scientific Launch Safe Series DNA Electrophoresis Products