Clear Bottom Assay Plates from Porvair Sciences

23 Aug 2015
Chelsie Phillips
Temporary Editorial Assistant

Product news

The unique Krystal 2000 microplate range from Porvair Sciences has been optimized for luminescence and fluorescence assays. A patented manufacturing process, using a ‘two-shot’ mould, provides clear, individual wells in an opaque matrix. The special design of the plate totally eliminates the well-to-well optical crosstalk inherent with other clear bottomed microplate designs, giving unmatched accuracy, sensitivity and repeatability of photometric readings. Very high signal-to-noise ratio and low detection limits can be achieved with this superb plate. All tissue-culture treated plates are supplied lidded and sterile, in individual bags.

Constructed in ultra-pure grade polystyrene, the opaque white 96-well Krystal 2000 plate maximises reflectivity enabling even weakly emitting luminescence assays to be routinely undertaken. Designed for luminescence applications that require the highest levels of photometric accuracy and repeatability the Krystal 2000 white microplate (well volume - 350 microlitres) incorporates a unique clear cup design that eliminates the performance-limiting well-to-well light crosstalk inherent in most other clear bottomed microplate designs. In addition to optimised luminescence measurements the unique design of the Krystal 2000 white plate offers improved cell binding efficiency and allows the convenience of direct measurements on bottom reading spectrophotometers and inverted microscopes.

Designed for fluorescence assays the black 96-well Krystal 2000 plate contains a significant quantity of carbon black, completely eliminating well-to-well cross talk by quenching all light penetrating the plate walls. The result is unmatched sensitivity and extended dynamic range across the whole plate, essential for cherry-picking assays where unknowns may be spread randomly across the plate.

Designed to conform to the standard ANSI / SLAS format - Krystal 2000 black and white plates are fully compatible with all commercially available top reading spectrophotometers, multi-mode readers, robotic sample processors and automated liquid handling systems.

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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.LuminescenceLuminescence techniques detect and measure light emitted by substances during chemical reactions or biological processes. Widely used in assays for diagnostics, environmental monitoring, and drug screening, luminescence-based methods offer high sensitivity. Explore leading systems and products in our directory.
Clear Bottom Assay Plates from Porvair Sciences