SelectScience
Join Free
Accelerating Science
NewsSeparations

Chromatography Solutions for Mass Spec

Phenomenex Inc
30 May 2011
bridget bridget
Laboratory Director

Product news

Visit Phenomenex in booth 121 to discover how our advanced technologies deliver better separations from sample preparation through final analysis. Attend our breakfast meeting for an in-depth look at Kinetex® 1.7 µm columns, the only sub-2 µm core-shell UHPLC column commercially available. Offering efficiencies and separation unmatched by all other UHPLC columns, Kinetex 1.7 µm columns allow one to get even higher throughput, shorter run times, and better resolution than existing UHPLC methods.

Expanding the Range of UHPLC Selectivity

Two recently introduced Kinetex phases expand the range of selectivity for chromatographers seeking ultra-high performance on any LC system. Kinetex XB-C18 gives superior peak shape and enhanced separation of basic compounds in acidic and neutral pH conditions. Kinetex C8 brings the benefits of core-shell technology to methods that require a USP L7 or other C8 column methods.

No LLE when Extracting Oligo Therapeutics from Tissues/Fluids

In less then 15 minutes and without the use of liquid-liquid extraction (LLE), scientists using Clarity® OTX™ to extract RNA & DNA-based therapeutics from biological fluids & tissues achieve high recoveries and LC/MS sensitivity, dramatically shortening the preclinical and clinical development timeline.

High Performance GFC/SEC Columns for Proteins and Peptides

BioSep™-SEC-S columns offer an optimized solution for Gel Filtration/Size Exclusion of protein and peptide samples covering all molecular weight ranges. These economically priced columns deliver extremely high efficiencies, exceptional column reproducibility, and up to two times the lifetime when compared to other GFC columns on the market.


Poster Presentations

• Rapid, Simultaneous Screen and Confirmation Analysis of Benzodiazepines in Biological Fluids by LC/MS/MS Using C18 Core-shell Column

Monday, June 6
Liming Peng

• Development of Tissue-Specific Isolation and Analysis Methods for Oligonucleotide Therapeutics and their Metabolites by LC/MS

Monday, June 6
Michael McGinley

• A Simplified and Streamlined Approach to Solid Phase Extraction for the New SAMHSA Drugs of Abuse Panel

Tuesday, June 7
Michael Rummel

Links

Phenomenex IncCompany website

Tags

AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  Capillary ElectrophoresisCapillary electrophoresis (CE) is used to separate molecules based on charge, size and hydrophobicity. Pressure, voltage or a vacuum is used to introduce the sample to fused silica capillaries. Fluorescence, UV/Vis, UV or diode array detectors are used to visualize components. Types of CE systems include capillary electrochromatography (CEC), capillary zone electrophoresis and capillary gel electrophoresis. Find the best capillary electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flash ChromatographyFlash chromatography is a fast-preparative column chromatography technique used for separation and purification of liquid analytes. Flash chromatography systems use small silica gel particles for separation and a pressurized gas for driving the solvent through the column or pre-packed cartridge. Find the best flash chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gas ChromatographyGas chromatography (GC) is an analytical technique used to separate and quantitate mixtures of small and volatile compounds. Gas chromatographs or GC systems include components such as GC columns, detectors, pumps and autosamplers. Choose from packed or capillary GC columns, flame ionization (FID), photoionization (PID) electron capture detectors and selective or non-selective detectors. Find the best gas chromatographs in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Supercritical Fluid ChromatographySupercritical Fluid Chromatography (SFC) is used for the rapid separation and analysis of complex mixtures. SFC systems require injectors, pumps, columns, ovens and detectors. The SFC retention method uses a supercritical fluid, usually CO2. There are a number of different SFC detector types including UV / VIs, ECD, mass spectrometry and fluorescence.ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.Medicinal ChemistryMedicinal chemistry is a broad discipline encompassing the design, identification, synthesis and development of chemicals in drug discovery. It includes a number of techniques covering structural biology, synthetic chemistry and molecular biology. Technologies used in medicinal chemistry include ADME, lab-on-a-chip, high content screening and assay assembly.VoltammetryVoltammetry is a sensitive analytical technique for elemental detection. In a voltammeter the current is measured in the system whilst the voltage is varied. Usually 3 metal electrodes are required including the reference, auxiliary and working electrodes. In polarography, a subclass of voltammetry, the working electrode used is a dropping mercury electrode (DME). The 2 main types of voltammetry are cyclic and linear sweep voltammetry.

Related articles

Chromatography Solutions for Mass Spec