ChemiDoc-It2 Gel/Blot Imagers from UVP

6 Oct 2015
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

UVP’s ChemiDoc-It2 Imagers feature an economical, modular design for chemiluminescent western blot and gel imaging research. Select the camera, light sources, emission filters and other components to configure a system for your applications and budget requirements.

  • Choose from cooled CCD cameras including the new MegaCam 815 camera with 8.1 megapixel resolution and high sensitivity for detecting the finest details in gels and blots.
  • Select from a variety of light sources including epi UV modules, multiple wavelength UV transilluminators as well as blue and white light plates for maximizing gel excitation
  • Add emission filters in the four position filter tray as needed for specific stains

The darkroom is light tight which is essential in low light detection of samples such as western blots. The blots are placed on the chemi tray; no film is required for western blots. View gels and blots through the UV safe gel viewing window. The wide front door and roll out tray provide easy access to the transilluminator and work area.

VisionWorksLS software loads on your computer for image capture and quantitative analysis. Capture functions include sequential integration which captures a series of pictures taken at increasing exposure times. AutoExpose tool automatically sets the perfect image exposure. Templates simplify workflow with user-defined and saved settings which can be recalled in repeat experiments.

Applications of the ChemiDoc-It2 system includes detection, capture and quantitative analysis of chemiluminescent blots (northerns, southerns, westerns), DNA and protein gels, blue light gels, multiplex multi-color gels and fluorescent western blots, TLC gels, colony plates and plants. Request information on configuring a ChemiDoc-It2 Imager to meet your application requirements.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Colony CountingGel ImagingGel imaging captures high-resolution images of electrophoresis gels to analyze DNA, RNA, and proteins. Essential in molecular biology, this technique helps visualize results for accurate quantification and documentation. Discover top imaging systems and compare user-reviewed options in our directory.CCD cameraChemiluminescenceUVUltraviolet (UV) light is used in a variety of applications, including disinfection, spectrophotometry, and molecular biology. UV light can damage DNA, making it a powerful tool in sterilization and in studies of mutagenesis and gene repair. Explore UV-related products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Blotting