Cellectricon Announces World's First System for High Throughput RNAi Screening

11 Sept 2007

Product news

Cellectricon, a leading provider of miniaturized & cell-based screening solutions for drug discovery, today announced collaboration with EPFL (École Polytechnique Fédérale de Lausanne, Switzerland). EPFL is evaluating Cellectricon's next-generation high throughput electroporation system. Cellaxess-HT is the world's first high throughput electroporation system tailor-made for genome wide RNAi screening and will be launched Q2 2008.

"We are pleased to collaborate with EPFL and look forward to bringing this state-of-the-art system to the market." says Dr. Mattias Karlsson, CEO at Cellectricon. "Cellaxess-HT will be the first of its kind on the market and will significantly impact drug discovery by opening the door to using more biologically relevant cell types in genome wide RNAi screening."

"We are impressed by the unprecedented high throughput delivery of genetic materials the Cellaxess-HT system offers. We believe that Cellaxess-HT has the potential to greatly advance our efforts in our RNAi research programs", says Dr. Gerardo Turcatti, Director of the Biomolecular Screening Facility at EPFL, Lausanne, Switzerland.

Cellaxess-HT addresses the critical need for high throughput delivery of genetic materials to hard to transfect cells in several cell-based screening assays. Cellaxess-HT will be the only fully automated system on the market allowing for high throughput electroporation of cells in culture directly in 384-well microplates. The throughput for the system is up to 50,000 wells per day and allows for genome wide screening with high transfection efficiencies and viabilities in cell lines as well as primary cell types.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.