Cell Sorting – A Roadmap to Best Practice

Join our webinar for a flow cytometry masterclass and learn the tools, tips and tricks to ensure superior cell sorting performance

1 Mar 2019
Matthew Mcardle

Expert insights

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Image of Cristina Ghirelli, immuno-oncology manager at Horizon Discovery
The webinar will be delivered by Dr. Peter Lopez, Director of Cytometry and Cell Sorting Laboratory, NYU Langone, and Dr. Rui Gardner, Director of Cell Sorting Laboratory, MSKCC

Flow cytometry-based cell sorting has become an indispensable and popular tool used to isolate cells for a wide variety of downstream applications, ranging from clonal expansion to single-cell genomics. As the technology behind cell sorting advances into chip-based systems, cell sorters are becoming easier to use, with researchers able to self-operate these systems.

If you are a research scientist new to cell sorting or simply want to improve the outcome of your sorting experiments, we invite you to join our discussion with two leading experts in the field – flow cytometry lab directors, Dr. Peter Lopez and Dr. Rui Gardner – and learn the path to superior sorting performance.

The speakers will discuss multiple topics that will help ensure sorting success for precious samples and share their recommendations for isolating single cells. The topics covered will include:

  • Best practices in cell sorting
  • Tips on how to optimally set up a cell sorter
  • A review of the cell sorting instrument characteristics that influence performance
  • Basic guidelines that can be applied to multiple different approaches for isolating your cells of interest

Key learning objectives

  • How purity, efficiency, and speed influence good sorting outcomes – and choices you’ll need to make to optimize sorting for your application.
  • How instrument characteristics can influence cell sorting performance – and how to select what will work best for your experiment.
  • How to set up for plate sorting to improve single cell deposition efficiency.

Who should attend the webinar

  • Those with any level of cell sorting experience using flow cytometry
  • New users of cell sorting technology looking for best practice guidance
  • Research scientists interested in optimizing their cell sorting experiments

Attend the live webinar
The live webinar will take place on Thursday, March 14, 2019 at:
• 16:00 GMT
• 12:00 EDT
• 09:00 PDT
• 17:00 CET

Interested, but have a scheduling conflict? Register to receive an on-demand link to watch later.

SH800 Cell Sorter

Sony Biotechnology

The benchtop SH800 cell sorter permits sorting of a wide range of cell sizes for many applications using the 70-µm, 100-µm, and 130-µm microfluidic sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to perform. System software is intuitive and supports sorting into tubes and 96- and 384-well plates.

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MA900 Multi-Application Cell Sorter

Sony Biotechnology

The MA900 from Sony meets the needs of most sorting applications, supporting 12 fluorescence parameters and 4-way sorting. Powerful, modern technologies built into the MA900 system include a patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability.

(19)

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell SortingCell sorting isolates specific cell populations based on physical or molecular characteristics, essential in immunology, cancer research, and regenerative medicine. Techniques like flow cytometry improve accuracy and speed. Compare cell sorting instruments and solutions in our product directory.