Cedars-Sinai experts discuss automated metabolite extraction from plasma

Watch this webinar with Prof. Jennifer Van Eyk to find out how to automate metabolite isolation from plasma, minimizing post-sampling variability and allowing for downstream LC-MS/MS analysis

8 Nov 2019
Arran Tabary-Davies
Microbiologist

Expert insights

Metabolites in blood exist in a state of dynamic equilibrium, making efficient sample processing and handling after blood sampling key to biologically relevant analyses.

Sebastian Weber
Left, Weston Spivia. Right, Prof. Jennifer Van Eyk

In this webinar, we discuss a method for evaluating room temperature quenching, lipid removal and metabolite isolation from plasma samples and how this protocol can be automated using the Agilent Bravo Metabolomics Sample Prep Platform.

Prof. Jennifer Van Eyk, Erika J. Glaer Chair in Women's Heart Health and Director, Advanced Clinical Biosystems Research Institute, and Weston Spivia, Research Associate III, Advanced Clinical Biosystems Research Institute, Cedars-Sinai, will share their work and discuss the benefits of this method, including its ability to minimize post-sampling variability and its compatibility with downstream LC-MS/MS analysis of metabolites.

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What you will take away:

  • Why efficient sample processing and handling after blood sampling is key to biologically relevant analyses
  • A method for plasma protein precipitation and subsequent metabolite extraction using the Bravo automated workstation can minimize post-sampling variability
  • How the automated plasma processing and metabolite extraction is compatible with downstream LC-MS/MS analysis of metabolites

Attend the live webinar on Wednesday, November 20, at:

  • 16:00 GMT
  • 11:00 EST
  • 08:00 PST
  • 17:00 CET

Who should attend?

Researchers involved in extracting and isolating metabolites from large numbers of plasma samples.

Find out more on this topic by registering to watch the full webinar>>

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AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.MetabolitesMetabolites are the intermediate and end products of metabolism. Analyzing metabolites offers insight into cellular processes and disease mechanisms and is critical in fields like pharmacology, toxicology, and diagnostics. Explore our peer-reviewed product directory to find the best tools for metabolite analysis, compare products, check reviews, and get pricing directly from manufacturers.PlasmaLC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.