Catch the “Buzz” with the New <i>Trans</i>IT®-Insect Transfection Reagent from Mirus Bio

16 Dec 2013
Sarah Thomas
Associate Editor

Product news

If low protein expression in insect cells is bugging you, consider the NEW TransIT®-Insect Transfection Reagent. Insect cells are a powerful platform for generating recombinant baculovirus and for producing high-levels of soluble, functional protein. TransIT-Insect is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types with distinct advantages over other reagents such as:

Exceptional DNA Delivery - In insect cell types including Sf9, High Five™ and S2
High Baculovirus Production - Ideal for baculovirus expression in insect cells
Serum Compatibility - Non-liposomal, animal-origin free formulation that eliminates media change
Better Value - Low reagent amounts required per transfection

“Many of the reagents addressing insect cell transfection are cationic-lipid formulations and yield low efficiency in serum containing media,” said Scott Hayes, Vice President, Mirus Bio LLC. “As our customers depend on the non-liposomal nature of TransIT® transfection technologies, they can be assured that TransIT-Insect will meet their expectations for high efficiency and robust protein production.”

In head-to-head testing, transfections with TransIT-Insect show at least 2-fold better protein expression in insect cells.

For experimental details and additional testing results, please visit www.mirusbio.com

TransIT®-Insect Transfection Reagent

Mirus Bio LLC

High yield transient transfection and baculovirus titers in insect cells. Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression.TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types.TransIT®-Insect Transfection Reagent Features: Exceptional DNA Delivery - In insect cell types including Sf9, High Five™ and S2 High Baculovirus Production - Ideal for baculovirus expression in insect cells Serum compatibility - Non-liposomal, animal-origin free formulation that eliminates media change Better Value - Low reagent amounts required per transfection

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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.