Carestream Molecular Imaging Releases Western Blot Kits For Added Convenience, Better Performance

24 Feb 2009
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Carestream Molecular Imaging, a division of Carestream Health, Inc., today released convenient, cost-effective kits for performing western blots — a popular analytical technique used to detect proteins. KODAK X-SIGHT Western Kits provide industry-leading components and step-by-step protocols designed to deliver peak performance from X-SIGHT Nanosphere Conjugates in western blots.

"The X-SIGHT Nanosphere Conjugates, when used with the components supplied in the Western Kit, outperformed our current western blot method," said Kevin McCarthy, Ph.D., Professor, Department of Pathology, LSU Health Sciences Center-Shreveport. "The whole process was faster, easier and the signal obtained was immediate, more stable and brighter—with less background noise."

KODAK X-SIGHT Western Kits are built on X-SIGHT Nanospheres, which are available in four distinct wavelengths including three in the near-IR range. The kits are offered in several different wavelength combinations that include up to three X-SIGHT Nanosphere Conjugates, making them ideal for multiplex applications. Each kit is optimized for 10 blots and includes best-in-class membranes and buffers to achieve high signal-to-noise ratios. A separate Accessory Kit is also available for use with any of the X-SIGHT Nanosphere Conjugates or any other commercially available fluorescently-labeled secondary antibody conjugate (nanoparticle or dye).

The new kit delivers added productivity and can reduce costs when compared with purchasing each component separately or in larger quantities than may be necessary. Components suppliers include Fisher BioReagents (Fairlawn, NJ) part of Thermo Fisher Scientific and Millipore Corporation (Billerica, Mass.).

The kits are available worldwide. U.S. customers can place orders directly or through channel partners.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Carestream Molecular Imaging Releases Western Blot Kits For Added Convenience, Better Performance