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Camena Bioscience uses gSynth to synthesize a 2.7kb plasmid

Camena Bioscience

Enabling new synthetic biology applications including protein production and the engineering of bacteria

10 Feb 2020
Sophie Ball
Publishing / Media

Industry news

Synthetic biology company, Camena Bioscience, has announced the achievement of another significant DNA synthesis technology milestone. Using gSynth, a novel multi-enzymatic de novo DNA synthesis and gene assembly technology, Camena Bioscience has produced a whole plasmid of 2.7kb.

The ability to read, write and manipulate DNA is essential for synthetic biologists to investigate and engineer biological pathways. Over the last 20 years, there have been significant developments in DNA sequencing (reading) and DNA manipulation technologies, but DNA synthesis has not improved at a similar pace. Previously, Camena Bioscience analysed the accuracy of gSynth by producing a series of 300 nucleotide DNA fragments and benchmarking them against phosphoramidite synthesis, which was the existing ‘gold-standard’ technology. This work highlighted the superior DNA synthesis accuracy of gSynth. However, the coding sequence of the average human gene is just over 1000 nucleotides long and therefore, to support the expanding needs of synthetic biologists, any new DNA synthesis technology must be able to produce long DNA molecules.

To demonstrate gSynth’s ability to produce long DNA molecules, Camena Bioscience accurately reproduced the DNA sequence of pUC19, a 2.7kb plasmid commonly used within the field. Plasmids are circular double-stranded DNA molecules that replicate within bacteria and are commonly used synthetic biology tools. Achieving this length of DNA synthesis is a significant milestone that demonstrates the utility of gSynth and enables a new range of synthetic biology applications, including the synthesis of genes for protein production and engineering of bacteria.

"While we have demonstrated gSynth is a highly accurate DNA synthesis technology, it was critical to show that it can also produce longer, gene-length, DNA molecules,” said Camena Bioscience CEO, Steve Harvey. “Our aim is to support the rapidly expanding synthetic biology market and this new milestone highlights how gSynth can enable the development of new and exciting synthetic biology products.”

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.Synthetic BiologyGenetic Engineering

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Camena Bioscience uses gSynth to synthesize a 2.7kb plasmid