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Bruker Announces Release of Breakthrough CaptiveSpray(TM) Nano/Capillary Electrospray Ion Source for Proteomics at ASMS 2011

Bruker Daltonics
9 Jun 2011
bridget bridget
Laboratory Director

Product news

At ASMS 2011, Bruker is introducing the breakthrough, proprietary CaptiveSpray electrospray ion source for nano-HPLC applications in proteomics. Using CaptiveSpray technology in many cases increases bottom-up protein identifications significantly, and CaptiveSpray is presently the best available technology for robust, reproducible protein ID or quantitative proteomics applications, with excellent, stable sensitivity over long time periods.

Unlike a traditional pulled nanospray tip, the Etch-Taper™ technology employed by CaptiveSpray ensures that the internal diameter of the spray tip remains constant, thereby reducing tip clogging, and providing excellent spray stability over the entire LC gradient and robust operation for long time periods, even with heavy proteomics samples loads. A key proprietary feature of the CaptiveSpray is its novel gas-flow focusing technology for dramatic sensitivity gains compared to normal electrospray. The CaptiveSpray source delivers nanospray sensitivity without the need for complex and time consuming spray tip adjustments, while its innovative plug-and-play design fits all current Bruker LC-MS instruments, including the latest maXis UHR-Qq-TOF systems, solariX FTMS systems and amaZon ETD ion trap mass spectrometers.

The novel CaptiveSpray source is uniquely designed to operate with LC flow rates from as low as 50 nL/min up to 5 uL/min and higher. This wide LC flow range allows analysts to significantly reduce column loading times, minimize column re-equilibration, and dramatically increase overall MS utilization time. The ability to run at higher LC flow rates, without compromising sensitivity, also increases sample loading capacity and expands the dynamic range of analysis. This combination of attributes provided by the unique CaptiveSpray source enhances the overall ability of the MS system to identify and quantify low-level peptides.

Dr. Kerry Nugent, Senior Vice President for Liquid Chromatography at Bruker's Chemical & Applied Markets Division, commented: "With the introduction of the CaptiveSprayion source, Bruker has taken another step to enhance our leadership position in proteomics. The ability of the CaptiveSpray source to deliver a robust consistent spray, regardless of gradient composition or flow rate, makes it the ideal high-sensitivity MS source for applications that require a high level of accuracy and precision, such as bottom-up proteomics, peptide ID and protein quantification."

For more information on CaptiveSpray and Bruker Daltonics please visit the company article page.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Electrospray Ionization

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Bruker Announces Release of Breakthrough CaptiveSpray(TM) Nano/Capillary Electrospray Ion Source for Proteomics at ASMS 2011