NewsSpectroscopy
Broad Range of Excellent Fluorescence Dyes for Coupling to Proteins and Nucleic Acids
1 Nov 2010Sarah Sarah
Marketing / Sales
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The available PromoFluor dyes (e.g. PromoFluor-488, PromoFluor-555, PromoFluor-590, & PromoFluor-647) from PromoCell span the wavelengths spectrum from blue to far red and include very cost-effective alternatives to well-known fluorophores.
These dyes show an outstanding photostability, a strong absorption, high fluorescence quantum yield and a very good water solubility. The intensity increases when coupled to biomolecules resulting in reduced influence of uncoupled dyes. PromoCell’s PromoFluor dyes are available as carboxylic acids, NHS esters and maleimides as well as conjugates with amino groups, biotin, avidin & streptavidin.
They are very useful for labeling proteins, antibodies and nucleic acids e.g. in immunofluorescence microscopy, FISH, proteomics and microarray applications. PromoKine also offers a wide range of high-quality PromoFluor-labeled secondary antibodies and phalloidins as well as ready-to-use protein, antibody and DNA labeling kits with different PromoFluor dyes at an excellent price/performance ratio. Phycobiliproteins (R-PE, B-PE and allophycocyanin) for fluorescent biomolecule labeling as well as the according conjugates and labeling kits are also available.
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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. 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Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Microarray AnalysisMicroarrays, also known as biochips, are used for the detection and analysis of multiple genes, proteins, antibodies, or biomarkers on a single microchip. This can reveal information on protein or gene expression, single nucleotide polymorphism (SNP), copy number variation (CNV), epigenetics and patient health in clinical diagnostic tests. Discover a range of microarray scanners and prefabricated antibody, protein, RNA and DNA microarrays for your analysis or consider creating your own custom microarrays with a microarray printer. Find the best microarray products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. 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