Bringing Lab Quality Biodetection Technology to the Field

Developing portable technology to detect high-consequence pathogens

7 Apr 2016
Sarah Thomas
Associate Editor

Editorial article

Learn how scientists are using the BioTek FLx800 Multi-Detection Microplate Reader to help develop biohazard detection platforms. 

SelectScience® spoke to Dr Gabriel Peckham, Principal Investigator, Black Ivory Biotech about his research into developing new transportable technologies for biodetection.

Black Ivory Biotech is focused on the development of “assays, software and electronic equipment for multiplex, low weight and power portable biodetection platforms”, which are “designed to be used by military, first responders and point-of-care technicians to detect high-consequence pathogens in people or the environment”, stated Dr Peckham.

Dr Peckham, whose previous work focused on anthrax detection, explained that the platforms are made to enable “high accuracy and sensitivity, but with a short sample-to-signal time,” an important consideration for the applications of this technology. “In these high-stress situations, it is important to have equipment that can be easily transported to the site of a potential threat without excessive weight and equipment and provide quality information quickly with minimal risk to the operator.”

Developing detection technologies for hazardous pathogens


We love this instrument, it is several instruments in one.

Dr. Gabriel Peckham  Black Ivory Biotech


“We perform a lot of commercially available antibody and DNA-based detection techniques to compare to our own methods.” Dr Peckham commented. “ELISA, lateral flow devices (aka immunochromatography strips), and PCR, are all used in addition to the techniques needed to develop our own technologies.”

As part of this research Dr Peckham uses the BioTek FLx800 Multi-Detection Microplate Reader to “evaluate antibody binding patterns, quantify proteins and DNA, determine conjugation efficiency and enzyme specific activity as well as running traditional ELISAs.” Dr Peckham highlighted that the “flexibility” and “all-in-one, one footprint” design of the FLx800 system were of benefit to his research.

Future perspective

Dr Peckham commented that research findings using these techniques will help to bring “lab-quality biodetection to the field, for the non-scientist” and aim to “make biodetection less expensive, easier to execute and more portable than the state-of-the-art.”

In the future Dr Peckham hopes to focus “product development in the biosciences” for which “a multi-functional microplate reader will always be a key lab instrument for several routine and some not so routine procedures.”

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Safety EquipmentLaboratory safety equipment is essential to the effective operation of all laboratories and is required to minimize the risk of harm during experiments. Safety equipment includes radiation safety, radioactive / hazardous waste disposal, alarms and personal safety.Pathogen DetectionPathogen detection is the process of identifying harmful microorganisms, including bacteria, viruses, and fungi, that can cause diseases. Accurate pathogen detection is critical for food safety, public health, and clinical diagnostics. Explore pathogen detection products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.