Biotium expands Cheetah Taq portfolio with new formulations for fast cycling PCR and proximity extension assays

Biotium, a leading provider of fluorescent reagents for life science research, proudly expands its trusted Cheetah^™ Taq DNA Polymerase line with two new formulations: Cheetah^™ FLEX HotStart Taq DNA Polymerase and Cheetah^™ PEA-PLEX HotStart Taq DNA Polymerase.

These optimized versions of Biotium’s chemically modified, antibody-free hot-start Taq offer unmatched activation speed and performance for modern workflows, including fast-cycling qPCR, proximity extension assays (PEA), and NGS-based high-throughput PEA for protein detection. While Cheetah^™ Taq has been a reliable choice for reducing non-specific amplification in PCR, these newly tailored formulations bring greater flexibility and targeted performance to meet the evolving needs of researchers and suppliers of high-throughput commercial assays.

Cheetah^™ FLEX HotStart Taq: Fast-cycling & PEA versatility

Cheetah™ FLEX is engineered for speed and adaptability across a range of applications. With a rapid 2-minute activation at 95°C, FLEX delivers up to 10x faster activation than AmpliTaq Gold® or HotStartTaq®, with none of the drawbacks of antibody-based hot-start enzymes. The antibody-free formulation eliminates animal DNA contamination and provides enhanced long-term stability. Cheetah™ FLEX is validated for fast-cycling PCR and qPCR-based PEA, making it a go-to choice for both routine and advanced workflows. After heat activation, Cheetah™ Taq has the same functional properties and fidelity as native Taq polymerase.

Cheetah^™ PEA-PLEX HotStart Taq: Optimized for NGS-based high-throughput PEA

Cheetah^™ PEA-PLEX is a unique formulation of Cheetah^™ Taq developed specifically for high-throughput, NGS-based PEA assay for protein detection. Third-party validated in high-throughput PEA, Cheetah^™ PEA-PLEX is also compatible with fast-cycling qPCR.

Key Features of Cheetah^™ HotStart Taq DNA Polymerase:

• Novel chemical hot-start mechanism: No antibodies, no activation lag
• Rapid 2-minute activation at 95°C
• Antibody-free, low-DNA enzyme for contamination-free results
• Superior long-term stability than traditional chemically modified Taq
• Versatile use for fast-cycling qPCR and PEA
• Cheetah^™ PEA-PLEX developed and validated for NGS-based PEA assays
• Available in ready-to-use kits and customizable bulk formats

Cheetah^™ FLEX and Cheetah^™ PEA-PLEX Hotstart Taq are available now and can be purchased in standard or bulk sizes. Both formulations are available for licensing; contact techsupport@biotium.com for evaluation samples and to discuss custom size, packaging, or formulation options.

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