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BioTek’s ELx405™ Microplate Bead Washer Accommodates Polystyrene and Magnetic Bead-Based Assays

Agilent Technologies
3 Dec 2009
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BioTek is pleased to announce that the popular ELx405 Microplate Washer is now available with vacuum filtration and biomagnetic separation modules to meet the growing demand for automated washing of microbead-based assays. Full 96- and 384-well microplate washing for bead-based assays, as well as standard ELISAs, is available for those who are incorporating various assay types.

The ELx405’s variable-range vacuum filtration module automates polystyrene bead washing for microplate-based assays developed with popular multiplex assay platforms along with filtration-to-waste processes. The biomagnetic separation module provides rapid and uniform separation of micrometer- and nanometer-scale magnetic beads for the newest generation of Luminex xMAP® assay platforms. Each can also be formatted to wash ELISA and cell-based assays.

Jason Greene, BioTek Product Manager, notes, “Many assays developed on the Luminex xMAP technology platform are available in polystyrene bead formats, and the trend is moving towards incorporating magnetic beads. Our engineers have created a solution for processing both bead types with optimized conditions for best performance.”

ELx405 models feature choice of keypad control or flexible Liquid Handling Control™ PC software, integration into most leading robotic systems including BioTek’s BioStack™ Microplate Stacker. Also available are a patented Dual-Action™ wash manifold, automated four-buffer switching, and patent-pending Ultrasonic Advantage™ for unattended wash manifold maintenance.

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Agilent BioTek ELx405 Select Deep Well Washer

Agilent Technologies

The Agilent BioTek ELx405 Select is the first deep well plate washer that aspirates and dispenses into 96- and 384-well deep well and standard height microplates. The ELx405 Select is available in several configurations to address a wide variety of plate washing applications, including ELISA and cell-based assays.

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Washing EquipmentWashing equipment is more convenient than ever before with automated systems, integrated and multi-application systems available. For instance microplate washers can be programmable, combined with reagents dispensers and used for applications such as ELISA, multiplex assays and PCR clean up.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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BioTek’s ELx405™ Microplate Bead Washer Accommodates Polystyrene and Magnetic Bead-Based Assays