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Bioo Scientific Releases NEXTflex™ 16S V4 Amplicon-Seq Kit for Bacterial Metagenomics Studies

5 Feb 2013

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The NEXTflex™ 16S V4 Amplicon-Seq Kit has been developed to simplify bacterial metagenomics studies using Illumina® HiSeq and MiSeq platforms. This kit allows users to go from sample to sequence in two hours, making it the fastest library prep kit available.

Using specialized NEXTflex primers that target the V4 region of the 16S subunit, a single PCR amplification simultaneously ligates the necessary sequencing and barcoded region for multiplexing. The entire workflow requires only one clean-up step maximizing recovery. Ideal for studies of microbiome community composition or comparative metagenomics, the NEXTflex V4 Amplicon-Seq Kit is available with up to 48 barcodes. Higher degrees of multiplexing are available on a custom basis.

The NEXTflex 16S V4 Amplicon-Seq Kit is the latest addition to Bioo Scientific’s line of innovative kits for next generation sequencing library preparation. The NEXTflex™ 16S V4 Amplicon-Seq Kit is available for next day delivery.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Metagenomics

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Bioo Scientific Releases NEXTflex™ 16S V4 Amplicon-Seq Kit for Bacterial Metagenomics Studies