Bioo Scientific Launches Barcoded Adapters for Sample Multiplexing in Next Generation Sequencing

Bioo Scientific has introduced AIR™ Barcoded Adapters which allow sample multiplexing in small RNA (ie. miRNA ) Next-Generation Sequencing reactions, while decreasing costs and increasing the number of samples that can be run simultaneously. miRNAs are small, single-stranded RNAs that regulate gene expression by partial complementary base pairing to specific mRNAs.

Recently it has been shown that these small RNAs regulate gene expression by targeting mRNAs for translational repression and degradation. miRNAs modulate many cellular pathways including growth and proliferation, apoptosis and developmental timing. Multiplexed Next-Generation Sequencing using Bioo Scientific’s AIR Barcoded Adapters will significantly speed up the discovery and analysis of these important molecules.

AIR Barcoded Adapters, designed to work with Illumina Next-Generation Sequencers, provide flexibility in high-throughput sequencing applications. They significantly increase scale and throughput while reducing costs by allowing the user to pool multiple library preparations into a single sequencing reaction. The AIR Barcoded Adapters enable the user to detect rare sample events amongst hundreds of samples, across several time points and even multiple genomes. Predesigned AIR Barcoded Adapters, are available in sets of 10, 20, or 30 and were designed based on uniform melting temperature (Tm) and sequences that have unique color space. Once attached to a sample, they may be pooled together and sequenced in a single flow cell run. Analysis post run allows the user to identify the sequence barcode back to specific samples. AIR Barcoded Adapters can also be used to multiplex Next-Generation Sequencing reactions for piwi RNA (piRNA), small nucleolar RNA (snoRNA) and other small, noncoding RNAs.

According to Dr. Masoud Toloue, Senior Scientist at Bioo Scientific, “Sample barcoding technology maximizes Next-Generation Sequencing by allowing sample multiplexing, large library generation, cross-genomic and gene-drug treatment studies. This tagged technology improves sequencing experimental design to such a magnitude; it is likely all future Next-Generation Sequencing runs will be performed with barcodes.”