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Bioo Scientific Launched the AIR™ DNA Fragmentation Kit for Genomic DNA Fragmentation

17 Apr 2011
bridget bridget
Laboratory Director

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Bioo Scientific continues to expand its line of kits designed to increase the flexibility of next generation sequencing library preparation with the launch of the AIR™ DNA Fragmentation Kit. The AIR™ DNA Fragmentation Kit offers a cost-effective procedure for the fragmentation of genomic DNA in next generation sequencing sample prep and has been optimized to physically shear uniform fragments of genomic DNA. This fragmentation step is a critical first step for next generation sequencing of genomic DNA.

The AIR™ DNA Fragmentation Kit was developed in collaboration with Eureka Genomics, a San Francisco bay area next-generation sequencing service provider which specializes in Illumina GAIIx sequencing and advanced bioinformatics analysis of sequence data. This collaboration allowed us to merge Bioo Scientific expertise in the development of unique library preparation solutions for next generation sequencing along with Eureka Genomics’ extensive experience as a service provider to improve traditional genomic DNA fragmentation protocol in a novel way. The AIR DNA Fragmentation Kit fragments DNA more efficiently than traditional nebulization protocols offering 30% greater DNA recovery than traditional fragmentation protocols. This kit contains nebulizers, a proprietary fragmentation buffer and complete instructions. AIR DNA Fragmentation Kit is designed to prepare libraries for either the Illumina/Solexa or the Roche 454 sequencing platform.

The AIR™ DNA Fragmentation Kit contains a redesigned nebulizer and optimized buffers and protocol that result in highly reproducible DNA shearing, with DNA fragment length distribution ideal for next generation sequencing library preparation. “The AIR™ DNA Fragmentation Kit is highly optimized and reduces the costs of library preparation while yielding consistent results“ said Dr. Heather Koshinsky, Chief Scientific Officer at Eureka Genomics.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

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Bioo Scientific Launched the AIR™ DNA Fragmentation Kit for Genomic DNA Fragmentation