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Bioo Scientific is Launching NEXTflex-96™ DNA Barcodes to Significantly Increase NGS Throughput

2 Oct 2011

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Bioo Scientific has announced the launch of the NEXTflex-96™ DNA Barcodes, which significantly increases next generation sequencing throughput by using barcoded indices to run multiple samples in parallel. The ability to increase the scale of next generation sequencing (NGS) is a critical requirement needed to make personalized sequencing a reality. Multiplexing is a big step in that direction.

The NEXTflex-96 DNA Barcodes allow users to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes accomplish this by providing 96 indexed adapters, each of which contains a unique 8 nt sequence. The indices are designed to be error resistant and allowing for proper differentiation between samples. Using the NEXTflex-96 DNA Barcodes, the index is added to the sample during the adapter ligation step, allowing PCR amplification to be reduced or eliminated, thus minimizing amplification-based bias and preventing poor reads from single base errors introduced during PCR. These barcodes can be used to multiplex genomic DNA, RNA and ChIP sequencing libraries.

According to Dr. Masoud Toloue, Bioo Scientific’s Director of Genomics Research, “The latest, second generation sequencing platforms have a great capacity for simultaneous re-sequencing of large numbers of samples, using short identifying index sequences and achieving adequate coverage. Using the NEXTflex protocol with built in double error correction, users can multiplex 96 samples and reduce day to day sequencing variability. We expect this to dramatically increase sequencing and gene capture throughput.”

The NEXTflex-96 DNA Barcodes have been optimized for use with the NEXTflex DNA Sequencing Kit but can also be used with other Illumina library prep protocols. Each set of barcodes has been functionally validated with Illumina GAIIx & HiSeq 2000 sequencing platforms. For people who want to multiplex but do not need 96 barcodes, Bioo Scientific offers flexible options with the NEXTflex™ DNA Barcodes which are supplied in sets of 6, 12, 24, or 48 unique barcodes.

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Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Microarray AnalysisMicroarrays, also known as biochips, are used for the detection and analysis of multiple genes, proteins, antibodies, or biomarkers on a single microchip. This can reveal information on protein or gene expression, single nucleotide polymorphism (SNP), copy number variation (CNV), epigenetics and patient health in clinical diagnostic tests. Discover a range of microarray scanners and prefabricated antibody, protein, RNA and DNA microarrays for your analysis or consider creating your own custom microarrays with a microarray printer. Find the best microarray products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.

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