NewsSpectroscopy
Bioanalytical methods for bacteria and protein characterization
10 Nov 2020
Finn Price
Administrator / Office Personnel
Expert insights
Molecular and cellular bioanalyses are increasingly moving towards faster and more sensitive approaches. Key techniques such as Raman spectroscopy, fluorescence spectroscopy, dynamic light scattering, nanoparticle tracking analysis and surface plasmon resonance imaging now allow for easy characterization all kinds of analytes, from cells to small molecules.
In this webinar, Nathalie Vollmer and Christelle Mégier from HORIBA Scientific will describe these different techniques and their application in life sciences and pharmaceuticals. The potential of these techniques will also be illustrated through different application examples for bacteria and protein analysis.
Key learning objectives:
- Expand your knowledge on particle characterization, Raman microscopy, fluoresence, SPRi
- Understand the concept behind the different optical technologies
- Get a feel for the quality of information obtained through use of the different techniques
- Gauge the benefits of these techniques for dedicated bacteria and protein characterization applications
Who should attend?
- Researchers working on bacteria and proteins in life sciences and pharmaceutical settings
- Users of particle characterization, Raman, fluorescence and SPRi
- Anyone who needs to characterize biological samples
The live webinar takes place on Tuesday, November 24, at:
- 15:00 GMT
- 16:00 CET
- 07:00 PST
- 10:00 EST
SelectScience runs 10+ webinars a month across various scientific topics, discover more of our upcoming webinars>>
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Atomic Absorption / Emission SpectroscopyAtomic absorption spectroscopy (AAS) and atomic emission spectroscopy (AES) — also called optical emission spectroscopy (OES) — are used to detect the elemental constituents in samples. Both techniques involve the atomization of a sample. Atomic absorption spectrometers may use a flame or furnace to create an atomic vapor of the sample before irradiation with spectral light. Optical emission spectrometers may use a flame, inductively coupled plasma (ICP), microwave plasma (MP) or spark arcs to atomize and excite the sample. At higher excitation energies, electrons can be emitted instead of photons, which can be useful for samples that can’t be atomized and for surface analysis. Explore electron spectroscopy equipment such as Auger spectrometers and photoelectron spectrometers for surface elemental analysis of samples. Find the best atomic absorption, photoelectron and optical emission spectrometers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Raman SpectroscopyRaman spectroscopy is used to discern the vibrational and rotational states of molecules and hence the chemical composition of a sample by measuring the inelastic scattering of monochromatic light. Explore a range of Raman spectrometers, including handheld/portable Raman spectrometers for QC/QA labs and in situ spectrometers for processes. Conduct Raman imaging for microanalysis of mixed samples using a Raman microscope. Raman spectrographs are also available. Find the best Raman spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Surface Plasmon ResonanceBacterial IdentificationProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.