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Bio-Rad’s New Immun-Blot® LF PVDF Membranes Improve Fluorescent Blot Detection

Bio-Rad
3 Oct 2011

Product news

Bio-Rad Laboratories, Inc. announces the launch of the Immun-Blot LF (low fluorescence) PVDF membranes; Protein blotting membranes optimized for fluorescence and multiplex fluorescence applications. The membranes offer high signal-to-background ratio, low autofluorescence, and superior protein retention to maximize blot detection sensitivity and enable downstream quantitation.

Two primary types of protein blotting membranes are used in western blotting applications: nitrocellulose and PVDF (polyvinylidene fluoride). While both types are suitable for colorimetric and chemiluminescent detection, their high autofluorescence and lower signal-to-background ratios are not suitable for fluorescent detection.

Immun-Blot LF PVDF membranes are a low-fluorescence alternative specifically designed for fluorescent immunoblotting and detection in the visible light (red/green/blue) spectrum. Immun-Blot LF PVDF membranes produce highly sensitive and quantitative results thanks to their superior signal-to-background ratios, which are three times higher than those of nitrocellulose and conventional PVDF.

Key Features
• Low autofluorescence and high signal-to-background ratio – produce a stronger signal, more accurate protein quantitation, and higher quality images
• Superior protein retention – offers binding capacity of 300 µg/cm2 and improves protein retention, especially of low-abundance proteins
• Precut membrane and filter paper sets – save time and eliminate excessive membrane handling and potential contamination

Immun-Blot LF PVDF membranes are compatible with any imager possessing visible RGB excitation channels, including Bio-Rad’s new ChemiDoc™ MP imager, which allows researchers to visualize proteins at every stage of a blotting experiment when paired with Bio-Rad’s proprietary Stain-Free technology. The membranes are also compatible with a wide range of fluorescent labels, such as DyLights, Q-Dots, and Alexa fluorophores, as well as colorimetric and chemiluminescent detection methods.

For product details visit the company article page.

Links

Bio-RadCompany website

Tags

Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Bio-Rad’s New Immun-Blot® LF PVDF Membranes Improve Fluorescent Blot Detection