Bio-Rad launches TrailBlazer Tag and TrailBlazer StarBright Dye label kits for antibody conjugation to StarBright Dyes
Newly released kits at SLAS2025 offer broad antibody compatibility and long-term stability for flow cytometry and western blot applications
28 Jan 2025Product news
Bio-Rad Laboratories will be showcasing its TrailBlazer™ Tag and TrailBlazer StarBright™ Dye Label Kits at SLAS2025 International Conference & Exhibition in San Diego, California (booth #534). The new kits offer a convenient method to label any antibody with one of Bio-Rad’s StarBright Dyes, for use in either flow cytometry or fluorescent western blot experiments.
Flow cytometry plot depicts human peripheral blood stained with antibodies against CD8 and CD4 to identify T helper and T cytotoxic cells. CD8 was labelled with SBV610 and CD4 with SBV440 using the TrailBlazer Antibody labelling Kit.
The new two-kit system provides researchers across academia, pharma, and biotech access to Bio-Rad’s StarBright Blue and StarBright Violet Dyes, employing Bio-Rad’s SpyTag and SpyCatcher technology to conjugate a dye to an antibody of choice. This enables the design and generation of bespoke, flexible high-plex panels for flow cytometry and easy multiplex integration for fluorescent western blots.
Bio-Rad’s range of StarBright Dyes, proprietary fluorescent nanoparticles conjugated to flow-validated antibodies against key immunological targets, offers researchers exceptional brightness with narrow excitation and emission profiles for precise resolution.
For fluorescent western blots, StarBright Blue 700 and 520, designed to work with the configuration of the ChemiDoc™ MP and ChemiDoc Go Imagers, are labeled to anti-mouse and anti-rabbit secondary antibodies, facilitating bright, highly sensitive fluorescent detection with short exposure times.
StarBright Dyes are compatible with most experimental protocols and flow cytometers, including the Bio-Rad ZE5 Cell Analyzer, without the need for special buffers, providing highly sensitive fluorescent detection, improving resolution of specific cell populations, and minimizing spillover and spreading. Bio-Rad's antibody team will also be exhibiting the ZE5 Cell Analyzer at SLAS2025.
“The new TrailBlazer Tag and StarBright Dye Label Kits are designed for researchers who require greater flexibility in their multiplex experiments, providing multiple label color palette options and access to a select range of our unique StarBright Blue and StarBright Violet Dyes, with additional dyes to be added to the range in the near future,” said Hilary Mavor, Marketing Director, Life Science Group, Bio-Rad.
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