Bio-Rad Launches Precision Melt Analysis Software, Enabling High-Resolution Melt Analysis on its CFX96 and CFX384 Real-Time PCR Systems

Bio-Rad Laboratories, Inc., a multinational manufacturer and distributor of life science research and clinical diagnostic products, today announced the launch of its Precision Melt Analysis™ software that enables high-resolution melt (HRM) analysis on Bio-Rad’s CFX96™ and CFX384™ real-time PCR systems.

HRM extends the usefulness of quantitative real-time PCR methodology to detailed post-PCR sequence analysis, enabling a broad range of applications such as mutation scanning, methylation analysis, and SNP genotyping. With its simplicity and abbreviated workflow, HRM analysis offers a cost-effective yet accurate alternative to other post-PCR genetic analysis techniques such as single-strand conformation polymorphism (SSCP), mass spectrometry, and sequencing.

Precision Melt Analysis software is a convenient and easy-to-use application that imports and analyzes data files generated from Bio-Rad’s CFX96 or CFX384 real-time PCR detection systems. Researchers can use the software to genotype samples based on the thermal denaturation properties of double-stranded DNA. They can also scan for new gene variants, screen DNA samples for SNPs, identify insertions/deletions or other unknown mutations, and determine the percentage of methylated DNA in unknown samples.

Precision Melt Analysis software works with the CFX96 and CFX384 systems to deliver sensitive and reliable detection for HRM applications. This new software package accommodates the needs of all HRM users, whether they are performing a single genotyping experiment or analyzing a comprehensive study comprised of multiple runs.

Precision Melt Analysis software allows the user to:

§ Streamline data analysis using the customizable default analysis settings

§ Use multiple data view options to manually assign sample genotypes by tailoring the software to the appropriate analysis

§ View multiple charts at once — with “Charts” view, the user can check all of the data charts, including the amplification plot

§ Increase analysis throughput — analyze multiple experiments from several melt runs together using the “Melt Study” feature.