Bio-Rad extends range of StarBright Violet and UltraViolet dyes for multiplex flow cytometry

StarBright Violet 760, StarBright UltraViolet 575, and StarBright UltraViolet 605 Dyes conjugated to highly validated antibodies for multicolor panel design in immunology research

10 Aug 2022
Rory Shadbolt
Publishing / Media

Product news

Bio-Rad Laboratories, Inc., a global leader in life science research and clinical diagnostic products, has extended its range of StarBright Dyes to provide greater flexibility in multicolor flow cytometry panels. The StarBright Violet 760, StarBright UltraViolet 575, and StarBright UltraViolet 605 Dyes offer improved brightness with narrow excitation and emission profiles for precise resolution.

The Bio-Rad range of StarBright Dyes provides researchers with highly stable, validated flow antibodies conjugated to proprietary fluorescent nanoparticles and specific to key immunophenotyping targets, supporting multiplex flow cytometry panels in immunology research. Minimal lot-to-lot variation ensures reproducible and consistent staining, and the dyes are resistant to photobleaching and do not lose signal in fixation. The dyes, excited by violet (405 nm) and ultraviolet (355 nm) lasers, are compatible with the Bio-Rad ZE5 Cell Analyzer and S3e Cell Sorter, as well as most flow cytometers and a variety of experimental protocols, without the need for special buffers.

“The continued expansion of the Bio-Rad StarBright Dye portfolio, currently totaling 17 dyes, nine of which are StarBright Violet Dyes, gives researchers greater flexibility and choice when designing flow cytometry panels, by providing high-performance dyes excitable at a range of wavelengths,” said Mike Blundell, PhD, Bio-Rad Product Manager, Flow Cytometry, Life Science Group. “The exceptional brightness and unique profile of the dyes delivers enhanced resolution of rare populations and low-density antigens.”

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Dyes
Bio-Rad extends range of StarBright Violet and UltraViolet dyes for multiplex flow cytometry