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Bio-Rad Announces New Oriole™ Fluorescent Gel Stain

Bio-Rad
3 Feb 2010
Sarah Sarah
Marketing / Sales

Product news

Oriole™ fluorescent gel stain is a general protein gel stain containing a dye that fluoresces when bound to proteins. Unlike other fluorescent protein gel stains on the market, Oriole stain features a simple, 90-minute staining protocol without fixing and/or destaining steps, allowing you to visualise your protein bands faster and easier. Oriole fluorescent gel stain is compatible with mass spectrometry and features higher sensitivity and dynamic range than Coomassie blue stain, making it a perfect tool for proteomics studies.

Features:
• Nanogram sensitivity
• 3 orders of dynamic range
• One-step, 90-minute protocol without the need for fixing and destaining
• Compatibility with enzymatic digestion and mass spectrometry

Oriole fluorescent gel stain has an excitation peak at 270 nm and emission peak at 604 nm, making it compatible with any UV-based imager such as Molecular Imager GelDoc™ XR+, ChemiDoc™ XRS+, VersaDoc™ MP imaging systems and EXQuest™ spot cutter from Bio-Rad.

Oriole fluorescent gel stain will not work with any laser-based imager systems such as Molecular Imager PharosFX™ system from Bio-Rad and Typhoon imagers from GE Healthcare.

Click on the Company article page link top right of this page to save 25% on your purchase of Oriole Fluorescent Gel Stain and other popular total protein stains.

Links

Bio-RadCompany website

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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Bio-Rad Announces New Oriole™ Fluorescent Gel Stain