SelectScience
Join Free
Accelerating Science
NewsSeparations

BAC BV Collaborates with Life Technologies to Provide New HPLC Affinity Columns

BAC B.V.
20 Jun 2011
bridget bridget
Laboratory Director

Product news

BAC BV, the leading provider of antibody-based affinity purification technology, has announced a strategic collaboration with Life Technologies, the global manufacturer of molecular diagnostic and research-use products, to bring the wide range of BAC’s CaptureSelect® affinity ligand specificities to Life Technologies’ POROS® chromatography media and pre-packed HPLC columns. This new suite of products will provide an unparalleled range of specificities and column formats for applications from discovery through to process monitoring.

The collaboration will create a range of ready-to-use HPLC columns pre-packed with CaptureSelect affinity ligands immobilized on POROS chromatography media. Designed for the high pressures and flow rates necessary to provide the real-time results demanded for biopharmaceutical quality assurance needs, the unique selectivity of these columns will support Process Analytical Technology (PAT) for virtually any biopharmaceutical manufacturing process.

“We are very excited to be able to provide a continuously evolving range of specificities and column formats that will allow more researchers to access the benefits of CaptureSelect technology”, commented Laurens Sierkstra, CEO of BAC. “Scientists will be able to routinely use CaptureSelect ligands in formats that support QbD approaches for process development and monitoring.”

Links

BAC B.V.Company website

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

Related articles

BAC BV Collaborates with Life Technologies to Provide New HPLC Affinity Columns