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Automated plate washing and vacuum filtration with Tecan's HydroFlex™ platform

Tecan
7 Nov 2006

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Tecan's new HydroFlex™ platform provides excellent performance, reliability and flexibility for a range of 96-well format applications, including washing of ELISAs, cellular assays and protein arrays, and vacuum filtration-to-waste, such as for PCR clean-up.

The platform's outstanding on-line control features set new standards for plate washers, with options including a process control (PC) manifold that monitors all wash steps, and a liquid level detection system (LLD) to monitor filling levels in wash buffers and waste bottles.

The compact and modular platform has very low residual volumes (under 2 ml) and multiple aspiration points for flat-bottom wells, ensuring consistent, high quality washing. The platform's gentle drop-wise dispense mode and movable wash head minimize cell detachment, even with weakly adherent cell-lines, and the advanced pumping system includes a dispense pump with RPM control and a double-head aspiration pump for reliable performance at low service costs.

The advanced vacuum filtration option benefits many applications, such as purification of DNA after PCR steps, where unwanted reaction by-products are easily removed by using filtration membrane plates that collect the purified DNA.

The HydroFlex platform is easily operated as a stand-alone instrument with its built-in keypad, or by using the intuitive HydroControl software for simple pre-programming of wash cycles and plate parameters. The software fulfils user administration requirements, including electronic records and signatures, and allows the HydroFlex to be integrated with Tecan's Freedom EVO® liquid handling workstations or Freedom EVOlyzer® ELISA analyzer.

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.

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Automated plate washing and vacuum filtration with Tecan's HydroFlex™ platform