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Automated KAPA Library Preparation on the ACSIA Platform

PrimaDiag

PrimaDiag provide automated library preparation for next-generation sequencing

4 Mar 2016
Weylan Kiam-Laine
Microbiologist

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Library preparation steps are long and repetitive. The automation of this preparation prevents any human error and improves the quality of the libraries thanks to greater repeatability and more accurate pipetting.

PrimaDiag developed their NGS system, based on the ACSIA platform, using the latest technologies, which allows the handling of magnetic beads, temperature control with 0.1°C of accuracy and shaking from 200 to 3000 rpm. This system fits with any kind of plate and deepwell available on the market (no skirted, semi skirted, skirted plates and so on). Moreover, all liquid handling parameters are carefully optimized for minimal reagent consumption and the protocols are optimized to minimize the number of tips used (3 tip boxes for preparing 48 libraries instead of 14).

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ACSIA Tips

PrimaDiag

PrimaDiag provides its own brand of tips “ACSIA Tips”. This tips are high quality and autoclavable and ensure a great pipetting quality for the pipettes or its platforms. Specially designed to meet the quality requirements of ACSIA line, are also compatible with many manual pipettes The ACSIA Tips are available in two packaging: Rack:96 tips per rack 10 racks per box Refill:96 tips per plate 5 plates per refill 10 refills per box

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ACSIA NGSLibPrep Edition

PrimaDiag

ACSIA NGSLibPrep Edition, the solution to automate the libraries preparation for next-generation sequencing. In view of increasing your productivity, avoiding contamination and improving your work efficiency, PrimaDiag created ACSIA NGSLibPrep Edition. It uses our software PrimaControlleur®II and has been designed to allow you to effectively perform your libraries preparation.ACSIA NGSLibPrep Edition allows you to perform 96 samples without human intervention libraries. To do so, it operates from just after the fragmentation up through the final PCR amplification. It is in this last step at the end of the process that a sufficient quantity of interesting fragments is obtained. Moreover, with our software and its innovative SMT technology, it ensures lower process costs by reducing the quantity of tips used during your manipulations. ACSIA NGSLibPrep Edition minimizes the use of your reagents and consumables during your processes and prevents waste. It also ensures minimal sample loss during treatment. At the end of your processes the yield with ACSIA NGSLibPrep Edition is increased by 20% over manual treatment and process costs are and highly reduced.To perform the libraries preparation, ACSIA NGSLibPrep Edition is available with 16 SBS positions integrating our patented Magnetic Work Unit and our heatingShaker 3000. This configuration is provided with a 250µL 1-channel pipetting head and a 250µL 8-channel pipetting head. Its’ PrimaController®II software offers both a realistic and interactive view as well as real-time assistance to increase your efficiency by letting you focus on your own tasks.Features: Magnetic Work Unit based on our patented APIM technology (Attraction Point by Induced Magnetization) Pipetting heads are interchangeable within seconds High efficiency automated platform managed by innovative, smart software Easy and User-Friendly programming Pre-programmed protocols for exome capture Add-in our protective enclosure for better safety during your processes One of the market's best quality/price values

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