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ArcticZymes Heat&Run® Ensures Complete Genomic DNA Removal from RNA Preparations

14 Aug 2012
Tesni Perry
Administrator / Office Personnel

Product news

ArcticZymes AS today announced the launch of Heat&Run®, a new RNA sample prep kit based on the company’s proprietary Heat-Labile (HL) dsDNase enzyme.

The heat lability and other properties of dsDNase enable Heat&Run® to overcome the assay-critical limitations of existing DNase-based techniques by rapidly and completely removing genomic DNA (gDNA) without damaging the delicate RNA. With a simple three-step mix-heat-run protocol, Heat&Run® is also designed for seamless integration into existing laboratory workflows.

According to Managing Director Jan Buch Andersen, Heat&Run® addresses a major market need in both qRT-PCR and high-throughput sequencing: “Removing gDNA with current DNase-based techniques is extremely time-consuming, involving multiple protocol steps and increasing the risk of sample degradation. This is particularly a problem in the quantification of low-copy transcripts or small samples. By contrast, the heat-inactivation of HL-dsDNase has been shown to be gentle enough to preserve your entire RNA population, including small RNA species. In addition, you no longer need to use labor-intensive resin columns, which are also hard to operation with small volumes. Similarly you can avoid adding EDTA that may interfere with your reactions downstream.”

The Heat&Run® kit comprises both buffers and HL-dsDNase and is suitable for use with any sample material. The Heat&Run® kit represents the first of a new range of kits to be launched from ArcticZymes based on the company’s proprietary enzymes isolated from Arctic sources.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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ArcticZymes Heat&Run® Ensures Complete Genomic DNA Removal from RNA Preparations