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Arbor Biosciences Launches CRISPR-Based Mitochondrial DNA Depletion Kits

Arbor Biosciences

New myNGS Guides MitoDeplete kits offer species-specific depletion of mtDNA from next-generation sequencing libraries

11 Jun 2019
Cameron Smith-Craig
Cameron Smith-Craig
Pharma and Applied Sciences Editor

Product news

Arbor Biosciences, a division of Chiral Technologies and a leader in developing molecular biology research tools, has announced the launch of myNGS™ Guides MitoDeplete™ Kits as an introduction to their growing catalog of guide RNA pools for targeted sequencing applications.

This new depletion system combines Arbor’s massively parallel, high-fidelity oligo synthesis platform with best-in-class Cas enzymes for efficiently targeting unwanted molecules from next-gen sequencing (NGS) libraries. In-stock MitoDeplete catalog kits have been specifically designed and validated for human and mouse applications, while kits for other custom species can be designed and synthesized on demand.

MitoDeplete kits are designed for use on any NGS library containing a significant percentage of mitochondrial DNA (mtDNA) molecules, such as ATAC-Seq (Assay for Transposase-Accessible Chromatin using Sequencing) libraries which typically contain an abundance of mtDNA-derived molecules. While modified ATAC-Seq protocols exist to reduce mtDNA, the techniques are more cost- and time-prohibitive, and can only be applied to newly-made libraries. CRISPR-based depletion with MitoDeplete delivers up to a 95% reduction in mtDNA-derived molecules in simple, single-step process, which translates into a savings of 30-70% in sequencing costs.

“The myNGS Guides MitoDeplete Kits improve efficiency in ATAC-Seq or any other library with a high percentage of mtDNA, via a one-step incubation to remove unwanted molecules,” stated Alison Devault, Director of Genomics at Arbor Biosciences. “We are excited to introduce this new tool so researchers can sequence what matters most in their precious samples.”

The Human and Mouse kits are available for immediate shipment in a 8 reaction kit size, wherein a single reaction is suitable for depleting an entire sequencing lane’s worth of libraries. Kits for custom species can be produced on request through collaboration with one of the product scientists at Arbor Biosciences.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.MitochondriaGenome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.CRISPRCRISPR technology enables precise editing of genes, allowing scientists to modify DNA at specific locations. This revolutionary tool is used in genetic research, drug development, and gene therapy. CRISPR has applications in agriculture, disease treatment, and creating genetically modified organisms (GMOs). Explore CRISPR solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Arbor Biosciences Launches CRISPR-Based Mitochondrial DNA Depletion Kits