SelectScience
Join Free
Accelerating Science
NewsLife Sciences

AnaSpec Now Offers 5-Hydroxymethyl Cytosine Monoclonal Antibody (Clone 4D9)

AnaSpec Inc.
20 Feb 2014
Sarah Thomas
Associate Editor

Product news

AnaSpec is pleased to announce the availability of a mouse monoclonal antibody specifically recognizing 5-hydroxymethyl Cytosine (5-hmC). This monoclonal antibody (Clone 4D9) is available in a 100 µg size. Alternatively, a starter kit containing 50 µg each of this antibody and the popular 5-mC (Clone 33D3) monoclonal antibody is also available. Together, these two monoclonal antibodies provide a fully qualified and guaranteed antibody pair for analyzing cytosine methylation pattern in Epigenetics research.

Methylation of cytosine to 5-methylcytosine (5-mC) is a major epigenetic event which interferes with the binding of proteins to DNA resulting in the exquisite control of gene expression and cell differentiation.

5-hydroxymethyl Cytosine (5-hmC)

5-mC can be further modified to 5-hydroxymethyl Cytosine (5-hmC) by the TET family of methyl-cytosine dioxygenases. 5-hmC is an oxidative product of 5-mC that appears to participate in several active DNA demethylation pathways. 5-hmC is a key marker related to pluripotency in embryonic stem cells and in cancer cells. Hence, this event may be important for normal processes, as well as during cancer cell development and progression.

Links

AnaSpec Inc.Company website

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Epigenetic AnalysisEpigenetic analysis products are used to study heritable phenotype changes that do not alter the DNA sequence, such as DNA modifications affecting gene activity. Determine whether genes have been methylated with DNA methylation quantification kits or methylation microarrays. Assess epigenetic regulation with histone modification kits or ChIP-on-chip microarrays. Kits are also available for bisulfite conversion and amplification of your samples, as well as epigenomic library preparation kits for next-generation sequencing (NGS). Find the best epigenetic products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

Related articles

AnaSpec Now Offers 5-Hydroxymethyl Cytosine Monoclonal Antibody (Clone 4D9)