AnaSpec Introduces SensoLyte® 520 Calpain Kit
19 Nov 2009Sarah Sarah
Marketing / Sales
Product news
AnaSpec has announced the release of the SensoLyte® 520 Calpain Activity Assay Kit, the industry’s first longest wavelength FRET based calpain assay. Optimized for detecting calpain activity, this kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate.
Cleavage of this FRET substrate generates two separate fragments. The subsequent release of 5-FAM fluorescence can be monitored at excitation/emission=490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (µ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.
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Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.