AnaSpec Introduces Recombinant Human BAFF Protein

BAFF (BlyS, TALL-1, THANK, TNFSF13B, zTNF4) belongs to the TNF superfamily of proteins and is expressed by myeloid cells. It is a 285-amino acid long type II transmembrane protein, with the soluble152-amino acid active region.1-4 BAFF plays a pivotal role in B-cells homeostasis including survival of immature B-cells, transition of B-lymphocytes from T1 to T2 stage, and causes increase of antibodies secretion by B-cells.

The sequence (Accession # NP_006564) corresponding to the extracellular domain of human BAFF along with a 6x His tag was expressed in E. coli. Recombinant human BAFF was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC) and refolded into its active state using a proprietary technique. The molecular weight of the recombinant human BAFF is 17.2 kDa. Containing less than 0.1 EU of endotoxin per 1 μg of protein as determined by LAL assay, the activity of BAFF was determined by its ability to stimulate production of immunoglobulin molecules such as IgG, IgA, and IgM in isolated mouse B-lymphocytes. Purified recombinant human BAFF is supplied as sterile and frozen condition at 0.1 mg/ml in 1 x PBS buffer with 5 mM DTT, pH=7.5.