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AnaSpec Highlights Calcein AM for Live Cell Staining

AnaSpec Inc.
16 Jan 2012

Product news

AnaSpec is pleased to announce the availability of Calcein AM at two purity grades, >90% or >95%, as determined by HPLC. AnaSpec also offers the SensoLyte® Calcein Cell Viability Assay Kit - a rapid and sensitive assay to detect viable cells. This kit provides ample reagents (dye and assay buffer) for 1000 assays in a 96-well plate format. AnaSpec’s Calcein AM, a green fluorescent cell viability indicator, has excellent quality, and is cost-effective.


Calcein AM (Calcein acetoxymethyl ester), a non-fluorescent, cell permeable compound, has been known to convert to the strongly green fluorescent calcein when hydrolyzed by intracellular esterases in live cells. It is widely used for determining cell viability since the fluorescence intensity of calcein is proportional to the amount of live cells. Calcein is a photostable reagent and is well retained in the cytoplasm of live cells and is not influenced by intracellular pH. Its bright green fluorescence can be monitored at Ex/Em=494nm /520 nm.

Amongst dye reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein AM is the most suitable indicator for staining viable cells due to its low cytotoxicity property and does not affect cell proliferation or viability. Calcein AM is used in cell invasion, adhesion, migration and other cell-based assays.
*Information on Calcium AM supplied by AnaSpec

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Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Green FluorescentCalcein AMCell InvasionCell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.

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AnaSpec Highlights Calcein AM for Live Cell Staining