AnaSpec Announces SensoLyte® Deubiquitination Assay Kits

AnaSpec, EGT Group, is pleased to introduce the SensoLyte® 440 and 520 Deubiquitination Assay Kits. The former kit provides a convenient assay for inhibitors/activators screening in deubiquitinylation or for continuous assay of enzyme activity. The latter kit, employs a peptide substrate conjugated to a green fluorophore, and is ideal for inhibitor/activator screening in deubiquitinylation or for continuous assay of enzyme activity.

A natural substrate, ubiquitin, coupled with the AMC (7-amino-4-methylcoumarin) fluorophore is used in the SensoLyte® 440 kit. Upon cleavage by a deubiquitin protease, this substrate generates a blue fluorophore that can be detected at excitation/emission=354/442 nm. The substrate supplied in the kit is exquisitely sensitive for ubiquitin hydrolases (UCHs). The detection limit can reach as low as 3.7 pg/mL.

Within the SensoLyte® 520 Deubiquitination Assay Kit from AnaSpec, bright green fluorescence is generated upon protease cleavage and can be detected at excitation/emission=490/520 nm. The long wavelength spectra of the green fluorophore released provides less interference from other reaction components.

For more information please see the company article page on the right.