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Analytical Protein Aggregate Separation Without a Column

Postnova Analytics GmbH
28 Mar 2017
Mia Harley
Biochemist

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Postnova Analytics announces the AF2000 MALS - a new temperature controlled Flow Field-Flow Fractionation - MALS system developed to provided unmatched separation, characterization and fractionation of biopharmaceutical proteins.

The AF2000 MALS employs a similar setup to a traditional GPC-MALS system but avoids the limitations of column-based chromatographic separations. In the AF2000 MALS system the liquid chromatography column is replaced by a proprietary separation channel technology that retains molecules based only on size and by the influence of a cross flow as a separation force. Because of the unique design of the system and the absence of any stationary phase, separation on the AF2000 MALS is achieved without exerting shear forces and stress on the proteins and aggregates being separated.

Incorporating Multi-Angle Light Scattering (MALS) and Asymmetric Flow Field-Flow Fractionation (AF4) the AF2000 MALS offers separation over a very broad molar mass and particle size range. With the additional benefit of being able to undertake precisely temperature controlled experiments - the AF2000 MALS is able to achieve separations with higher resolution, reproducibility and recovery then has previously been possible.

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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Field Flow FractionationMALS

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Analytical Protein Aggregate Separation Without a Column