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An Excellent Range of Anti-human PGP 9.5 Antibodies

AbD Serotec
10 May 2007

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Protein Gene Product 9.5 (PGP 9.5), also called Ubiquitin Carboxyl-terminal Hydrolase (UCH) Isozyme L1, is a neuronal UCH isoenzyme involved in the regulation of the ubiquitin pathway.

There is evidence that disorders such as Alzheimer’s Disease (AD) and Parkinson’s Disease (PD) are related to the accumulation of ubiquitin caused by a mutation or reduction in PGP 9.5. In particular, a mutation in PGP 9.5 has been directly associated with the early onset of familial PD, and the protein is also down-regulated in idiopathic PD. Reduced PGP 9.5 function may also jeopardize the survival of neurons, which maybe another causative factor of these diseases.

AbD Serotec offers a wide range of reagents specific to PGP 9.5, including our rabbit anti-human PGP 9.5 antibody, 7863-0507. This antibody stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibers in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas, and tumors of the diffuse neuroendocrine system. It is paraffin-reactive and also suitable for Western blotting. As well as being a marker for human PGP 9.5, this antibody can also identify the protein in rodents, pig, sheep, dog and cat.

Our mouse anti-human PGP 9.5 antibody, 7863-1004, is a monoclonal alternative. Again it is paraffin-reactive, showing similar staining patterns to the polyclonal, but less cross-reactivity with other species. It can also be used for ELISA assays.


References:

Choi, J. et al. (2003) J. Bio. Chem. 279:13256-13264
Drapkin, P. T. et al. (2002) BMC Dev. Biol. 2:1-11
Lam, A. Y. et al. (2000) PNAS 97:9902-9906
Lombardino, A. J. (2005) PNAS 102:8036-8041

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.

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An Excellent Range of Anti-human PGP 9.5 Antibodies