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AMSBIO Offers Wnt Signaling Pathway Research Products

AMSBIO
5 Oct 2016
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

Anglo-American life science firm AMSBIO offers a comprehensive selection of products for Wnt signaling research including recombinant proteins, stable reporter cell lines, polyclonal antibodies and ELISA assay kits.

Wnt proteins are a family of cysteine-rich secreted polypeptides involved in important cell functions including cell to cell communication, cell proliferation, cell migration, cell polarity, survival and self-renewal. In particular Wnt3a signals play an important role in the ability of organoids to expand. Additionally, loss of activation of Wnt expression has been associated with alteration of cell fate, morphogenesis and mitogenesis which has considerable relevance in developmental biology and oncology research.

AMSBIO has introduced a new range of Wnt human and mouse recombinant proteins available in both high (85 - 90%) and lower (75%) purities. Suitable for various cell based assays and treatments, the human recombinant proteins are purified from HEK293 cells while the mouse proteins are expressed in CHO cells. In addition, AMSBIO also offers a range of Wnt stable reporter cell lines and polyclonal antibodies. The HEK293, HCT-116 or SW480 Wnt stable cell lines all have available matching controls and can be ordered also as a package in pairs. AMSBIO polyclonal Wnt antibodies are produced in-vivo and are suitable for ELISA, IHC and Lateral Flow applications. For detection of several different Wnt proteins, AMSBIO offers several easy-to-use and sensitive human and mouse ELISA assay kits.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody ArraysAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Developmental BiologyCell SignalingCancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

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