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AMSBIO Introduces ALiCE - A New High-Yield Cell-Free Protein Expression System

AMSBIO
6 Dec 2018
Holly McHugh
Administrator / Office Personnel

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Cell-free protein expression (CFPE) is today used by protein chemists to quickly produce small amounts of proteins when screening DNA libraries. However current technologies are limited and there is a need for higher protein expression yields.

ALiCE® is a unique eukaryotic system, which yields an unprecedented 3 mg/ml of protein in batch mode. Even prokaryotic systems, which generally can only handle easy-to-express proteins, only get at their best 1 mg/ml protein in batch mode.

These higher production efficiencies achieved by ALiCE™ make it possible for biopharmaceutical researchers to detect a significantly higher number of candidates from each DNA library screened, eventually leading to a higher number of viable candidates for development. ALiCE™ also offers the option to produce larger quantities of proteins.

Alex Sim, CEO of AMSBIO commented “Leading-edge protein research demands speed and robust technology. The cell-free approach offered by ALiCE™ enables high-quantity protein to be produced and validated in days instead of weeks. He added "ALiCE™ is also superior to other commercially available CFPE systems in terms of protein yield, making it ideal for the discovery of new protein drugs".

The ALiCE™ cell-free protein expression system was originally developed by Dow AgroSciences in collaboration with the Fraunhofer Institute for Molecular Biology and Applied Ecology IME (licensed by LenioBio) and is already being successfully used in agricultural research.

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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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AMSBIO Introduces ALiCE - A New High-Yield Cell-Free Protein Expression System