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AMSBIO Announces Non-Animal Recombinant Proteins for Cell Culture and Tissue Engineering Applications

AMSBIO
29 Apr 2010
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AMSBIO has announced a new range of recombinant proteins that can substitute extracellular matrix proteins that are currently extracted from mouse sarcoma.

The Mussel Adhesive Protein based matrix (MAPTrix®) ECM line of products are coating reagents with genetically incorporated bioactive peptides which mimic the extracellular matrix (ECM) activity of collagen, fibronectin, laminin, and vitronectin proteins. These bioactive peptides mimic ECM proteins and provide for cell attachment, spreading and growth. A MAPTrix® surface coating offers an animal-free, chemically defined attachment and growth surface that provides a highly controlled environment for cell culture and tissue engineering applications.

Cell adhesion to the extracellular matrix is essential for the development and maintenance of cells. Cell adhesion to ECM ligands is primarily mediated by integrins. The Arg-Gly-Asp (RGD) recognition motif is present in many ECM proteins including fibronectin, laminin, and osteopontin. Many biomimetic approaches have sought to convey biofunctionality to synthetic materials by presenting a cell adhesion motif such as the RGD recognition motif. The immobilization of short peptides such as RGD on synthetic or natural materials may produce biofunctional surfaces that bind adhesion receptors and promote cell adhesion; however, this conventional chemistry approach is time consuming, inconvenient, and lacks reproducibility.

The MAPTrix® ECM line of products provide a superior alternative to the conventional chemistry approach. In addition to RGD motifs - MAPTrix includes a wide variety of alternative motifs that mimic the extracellular matrix (ECM) activity of collagens, fibronectin, laminin, vitronectin and other matrix proteins. The use of MAPTrix® ECM based coatings is simple, convenient and highly reproducible. The MAPTrix® ECM line of flexible products can be used in your cell culture application alone or in combination with other products. All MAPTrix® products are soluble in a variety of buffers including water under a wide range of pH (pH= 2.0 - 9.5). Available for basic research or drug discovery applications in serum-free or serum-reduced conditions - MAPTrix® products are provided as ready-to-use aqueous coating formulations in 1.0 mg, 2.5 mg, 5.0 mg and 10.0 mg sizes (vials). For further information visit the AMSBIO website or contact AMSBIO via telephone or e-mail.

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Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Medicinal ChemistryMedicinal chemistry is a broad discipline encompassing the design, identification, synthesis and development of chemicals in drug discovery. It includes a number of techniques covering structural biology, synthetic chemistry and molecular biology. Technologies used in medicinal chemistry include ADME, lab-on-a-chip, high content screening and assay assembly.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.

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AMSBIO Announces Non-Animal Recombinant Proteins for Cell Culture and Tissue Engineering Applications