SelectScience
Join Free
Accelerating Science
NewsLife Sciences

AMSBIO Announces New Next Generation Sequencing Library Sample Preparation Kits

AMSBIO
27 Oct 2014
Sarah Thomas
Associate Editor

Product news

AMSBIO announces RapidSeq – new convenient and cost-effective library sample preparation kits for Next Generation Sequencing (NGS) research and diagnostic work.

The RapidSeq Small RNA Sample Prep Kit is optimized for preparation of NGS libraries for subsequent cluster generation, using purified mRNA or total RNA which contains small RNA fragments as input. The protocol includes steps for adapters ligation, reverse transcription, PCR amplification, and size selection by gel purification to generate a library product compatible with a range of commercial NGS platforms.

The RapidSeq Directional mRNA Sample Prep kit provides strand-specific cDNA synthesis with reduced costs and increased sensitivity. Strand-specific RNA-seq improves on standard RNA-seq in three ways: accurately identifying antisense transcripts, determining the transcribed strand of non-coding RNAs (e.g. lincRNAs), and demarcating the boundaries of closely situated or overlapping genes.

In addition using RapidSeq kits your workflow will be simplified as most components are supplied as ready-to-use master mixtures reducing your setup time and liquid handling steps. Offering market-leading performance and a wide dynamic range - RapidSeq provides a turnkey solution for applications including mRNA detection and quantification, mRNA discovery, mRNA expression profiling as well as mRNA related functional assessment and validation.

Links

AMSBIOCompany website

Tags

Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.RNA-SeqRNA sequencing (RNA-seq) is a next-generation sequencing technique used to analyze the transcriptome, providing insights into gene expression and regulation. This method is essential for genomics, disease research, and personalized medicine. Explore RNA-seq tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

Related articles

AMSBIO Announces New Next Generation Sequencing Library Sample Preparation Kits