amaxa’s New Nucleofector® 96-well Shuttle™ System for High Throughput Transfection

Today, efficient and reproducible high throughput transfection remains a challenge. Using amaxa’s 96-well Shuttle™, a high throughput extension for the well established Nucleofector® platform, transfection of difficult-to-transfect cell lines and primary cells is no longer out of reach.

The 96-well Shuttle™ is a high throughput transfection system based on amaxa’s well established Nucleofector® technology. Designed for applications such as RNAi based target identification and validation as well as reporter gene assays, it combines high-throughput capabilities with Nucleofector technology benefits.

This highly efficient, non-viral gene transfer method, suitable for primary cells (e.g. T cells) and hard-to-transfect cell lines (e.g. HL-60) is based on electroporation and uses a combination of optimized electrical parameters and cell-type specific solutions.

The Nucleofector 96-well Shuttle™ System is suited for the high throughput transfection of DNA, siRNA duplexes, and shRNA using the same transfection protocol. Transfection efficiencies of up to 90% with DNA and up to 99% with siRNA duplexes are achieved.

The system uses disposable modular standard 96-well Nucleocuvette™ plates. Each well can be addressed individually, i.e. 96 different transfection conditions can be tested in one plate, truly accelerating the optimization process.

By extending its sophisticated Nucleofector technology, amaxa sets a new standard for high throughput transfection, addressing both industrial and academic researchers.

For more information about amaxa’s new 96-well Shuttle, visit booth #2153 at AACR in Washington. Alternatively e-mail scientific-support.US@amaxa.com or visit www.amaxa.com/96well