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AIR™ Sample Prep Kit for Illumina-Compatible Small RNA Sequencing Launched

17 May 2010
Sarah Sarah
Marketing / Sales

Product news

Bioo Scientific announces the availability of the patent pending AIR™ Small RNA Sequencing Kit for preparation of Illumina-compatible small RNA sequencing libraries. The AIR Small RNA Sequencing Kit offers many advantages over existing small RNA sample prep protocols. It has been optimized for sample multiplexing using the AIR Barcoded Adapters, which decrease costs by increasing the number of samples that can be run in a single flow cell without introducing bias into the reaction.

The AIR Small RNA Sequencing Kit includes AIR Ligase, an enhanced enzyme that allows greater sequencing coverage than currently available T4 RNA ligases. Additionally, adapter sequence contamination is decreased and PCR band separation improved. The kit contains all of the enzymes, buffers, adapters and primers needed for sequencing on Illumina sequencing instruments.

According to Dr. Masoud Toloue, Senior Scientist at Bioo Scientific, “The discovery and sequencing of novel non-coding RNA (such as microRNA) has coincided with a wealth of functional validation and expression studies, making this an exciting time for transcriptome research. In order to understand the significance of global small RNA profiles and the importance this may have for future clinical applications, multiplexed small RNA studies will be a must. Bioo Scientific launched the AIR Small RNA Sequencing Kit to meet these demands.”

The AIR Small RNA Sequencing Kit is the latest addition to Bioo Scientific’s AIR next-generation sequencing line, which includes the AIR Barcoded Adapters, Custom AIR Adenylated Adapters and AIR Ligase. The response from early adopters of the AIR next-generation sequencing technology has been very positive.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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AIR™ Sample Prep Kit for Illumina-Compatible Small RNA Sequencing Launched