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Agencourt<sup>®</sup> Chloropure System Isolates DNA and RNA From Plant Material

Beckman Coulter Diagnostics
13 Feb 2008

Product news

Beckman Coulter, Inc. introduced the Agencourt® Chloropure System at the Plant and Animal Genome (PAG) and LabAutomation2008 Conferences last month. This new extraction kit for DNA and RNA isolation and purification from plant material delivers significantly higher recovery than other commercially available systems. The Agencourt Chloropure chemistry is automated on the Beckman Coulter Biomek® NXP and FXP Laboratory Automation Workstations to process three 96-well plates in just 80 minutes.

At the recent Beckman Coulter workshop held at the PAG conference, David W. Still, Ph.D., of California State Polytechnic University, Pomona, described his work in high-throughput gene expression profiling of lettuce and other composites. He presented a study comparing Agencourt Chloropure with another 96-well extraction kit. The results were verified using both the Beckman Coulter GenomeLab GeXP Genetic Analysis System and real-time qPCR*. "Our comparative genomics work requires huge numbers of genes and samples, and we must use high-throughput methods," explained Dr. Still. "With the Agencourt Chloropure chemistry, automated on Beckman Coulter robotics, we got great RNA preps with high yields and there was no contamination with genomic DNA or organic solvents."

Agencourt Chloropure incorporates the patented Agencourt SPRI® (Solid Phase Reversible Immobilization) paramagnetic bead-based nucleic acid purification technology, which does not require organic solvents, vacuum filtration or centrifugation steps. A complete turnkey solution is also offered, incorporating Beckman Coulter's sample preparation technology and GenomeLab GeXP Genetic Analysis System.

"Plant research is a growing field worldwide with users in agri-biotech, biofuels and academia," commented Todd Lombardo, senior product manager for Agencourt. "The Agencourt Chloropure system provides an ideal solution for downstream PCR applications."

Agencourt Bioscience Corporation, a wholly owned subsidiary of Beckman Coulter, is a leading provider of nucleic acid purification products and genomic services for life science research. The company's products and services are based on the patented Agencourt SPRI (Solid Phase Reversible Immobilization) paramagnetic bead-based technology.

*The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Agencourt<sup>®</sup> Chloropure System Isolates DNA and RNA From Plant Material