Affymetrix and Leica Biosystems Announce the Launch of Fully Automated RNA <i>in situ</i> Hybridization Assays

ViewRNATM eZ Assays, fully automated on the Leica BOND RX, offer remarkable sensitivity and specificity of staining for coding and non-coding markers in an easy-to-use, fully automated format.

Affymetrix, Inc. and Leica Biosystems announce the launch of Affymetrix’ fully automated RNA in situ hybridization assays ViewRNATM eZ Assays on the Leica BOND RX staining platform. These assays detect coding RNA as well as novel non-coding markers in formalin-fixed paraffin-embedded (FFPE) tissues. Dedicated for research use only*, the assays provide researchers with a powerful automated solution for drug discovery, translational research, and the development of new diagnostic tests for personalized medicine. The automation significantly reduces hands-on time and assay run time for profiling single-cell gene expression.

The Leica BOND RX system automates the staining process, bringing all the benefits of consistency, reduced labor, and speed. This fully automated assay is hands-free from tissue de-waxing to counter staining, and offers high sensitivity, specificity, and reproducibility with easy standardization across laboratories. ViewRNA eZ Assays represent a major technological advancement offering a robust and reliable platform to easily interrogate the function and disease relevance of any expressed genes.

“In particular, the ability to target non-coding as well as coding transcripts opens up a significant new capability to discover powerful new biomarkers for molecular test development, as the importance of the non-coding regions of the transcriptome are becoming more and more evident,” said Andrew Last, PhD, Chief Operating Officer at Affymetrix. “In addition, researchers can now design probes to visualize RNA transcripts coding for secreted proteins, which are important targets that traditional immunohistochemistry assays have challenge detecting.”

Micah Litow, Director, Research & Innovation, Advanced Staining Instruments at Leica Biosystems added: “Leica is very excited that the BOND RX is fully automating the Affymetrix ViewRNA eZ Assays. We are proud to have put such a powerful tool into the hands of the dedicated researchers who are making important discoveries, increasing our knowledge and improving cancer diagnostics.”

Affymetrix’ ViewRNA eZ Assays on the Leica BOND RX platform are now available for research applications. The ViewRNA eZ staining protocol is pre-loaded onto the Leica BOND RX, and the staining kit optimized for FFPE samples and probes of choice are available from Affymetrix.
*For Research Use Only. Not for use in diagnostic procedures.