SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Achieve fast and reliable detection of fully assembled AAV capsules with PROGEN Dip’n’Check AAV tests

PROGEN

The AAV lateral flow tests represent a quick in-process control suitable for crude lysate as well as purified AAV analysis within less than 20 minutes

25 Mar 2022
Blake Forman
Content Creator

Product news

Adeno-Associated Virus (AAV) vectors have developed to be one of the most commonly used vectors for gene transfer, representing a promising therapeutic approach to target inherited, genetic diseases, and many others. The gene therapy community, attempting to find treatments for a variety of genetic disorders faces several obstacles such as meeting the requirements from regulatory authorities as well as the time pressure caused by the rapidly growing market. Though the use of orthogonal methods is recommended for comprehensive characterization, rapid testing of individual and crude AAV samples during the development and manufacturing process is often difficult. Most precise analytical AAV tools are not convenient for single sample measurements and/or require highly purified samples.

PROGEN´s mission is to provide high-quality products that help advance new therapies and develop existing research processes safely, quickly, and affordably for the gene therapy community. For this reason, the PROGEN team developed the Dip´n´Check AAV kits, based on PROGEN’s expertise in AAV assay development and the urgent need for fast analytical methods in the growing field of AAV-based gene therapy.

The Dip´n´Check AAV lateral flow tests allow fast in-process control at every step of your process and can be used for the analysis of crude lysates as well as purified AAV samples. The kits have been developed by PROGEN based on its unique AAV particle antibodies which detect fully assembled AAV particles and allow the estimate of the actual AAV titer in your sample. These antibodies have widely been proven to be a suitable and reliable tool for the detection of intact AAV capsids.

Possible applications of the Dip’n’Check lateral flow tests include:

Comparison of AAV titers of different AAV preparations to e.g.

  • Select the most promising sample for further processing

    • Estimation of AAV titers in crude and purified AAV preparations to e.g.

  • Control efficiency of your processes
  • Pre-test AAV concentrations to hit the dynamic range of downstream analytics

    • Testing the binding of common AAV antibodies to an AAV capsid variant to e.g.

  • Validate suitability of antibody-based methods for further analysis

    • The rapid, semi-quantitative analysis of the Dip´n´Check AAV kits facilitate the acceleration of decision processes and help save precious time and sample in less than 20 minutes.

    Just follow the simple 3-step process:

    Prepare your sample by adding the included antibody solutions

    Incubate for 10 minutes

    Add the lateral flow test stripe and get your result in just 10 minutes

    Increase the probability for success and establish how to proceed best in the process early on.

    Key features:

    • Results in less than 20 minutes
    • Suitable for analyzing crude lysate and purified AAV samples
    • Ideal in-process control for purification processes for example
    • Pre-testing AAV concentrations for downstream analytics
    • Available for AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, and AAV9

    Want the latest science news straight to your inbox? Become a SelectScience member for free today>>

    Related products

    Request Quote for All Products

    Links

    PROGENCompany website

    Tags

    ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Lateral Flow AssaysBiotherapeuticsBiotherapeutics are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or in <i>in vivo</i> diagnostics. The most well-known example of a biotherapeutic product, and the first to be approved for therapeutic use, was recombinant human insulin.VirusesViruses are microscopic pathogens that require a host cell to replicate. Understanding their structure, replication cycle, and impact on the immune system is crucial in developing effective treatments, vaccines, and diagnostic methods. Research continues to focus on emerging viral diseases and antiviral drug discovery. Explore solutions for virus research, detection, and treatment in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Gene TherapyGene therapy is a technique that modifies or replaces genes within an individual's cells to treat or prevent diseases. This approach holds potential for curing genetic disorders, cancers, and certain viral infections. Advances in gene editing technologies like CRISPR are driving the growth of gene therapy. Explore gene therapy tools and technologies in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

    Related articles