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Abcam Introduces Alexa Fluor® 680/790 Secondary Antibodies for Fluorescent Western Blotting

Abcam plc
3 Mar 2014
Sarah Thomas
Associate Editor

Product news

Abcam plc, a global leader in the supply of innovative protein research tools, announced today it has added Alexa Fluor® 680/790 secondary antibodies for fluorescent western blotting to its existing Alexa Fluor conjugated antibody range.

Produced and extensively validated in Abcam laboratories, the F:P ratios of Alexa Fluor 680/790 have been optimized to ensure high quality and performance. The expanding range offers a broad portfolio of labeled secondary antibodies with guaranteed bright staining and low background to meet the different needs of researchers undertaking multi-color analysis.

High-performance antibodies for fluorescent western blotting

Brian Carpenter, Product Manager, Secondary Antibodies, Abcam, commented: “The popularity of fluorescent western blotting is growing and presents many advantages over the traditional method. With no need for film and chemiluminesence, fluorescent western blotting is more convenient and enables greater signal stability and the ability to facilitate simultaneous multi-color analysis on one blot. High performing antibodies are crucial for fluorescent western blotting and we’re pleased that our new Alexa Fluor680/790 secondary antibodies can support this trend.”

Ideal for multi-color staining
Abcam Alexa Fluor secondaries are ideal for scientists undertaking multi-color staining from all research backgrounds. The addition of these products complements Abcam’s innovative RabMAb® primary products and market leading primary antibody range.

Abcam Alexa Fluor secondaries are supported by expert scientific support, information-rich datasheets, and are available globally with fast delivery either directly through the Abcam website or via one of Abcam’s authorized distributors.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Secondary Antibodies

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Abcam Introduces Alexa Fluor® 680/790 Secondary Antibodies for Fluorescent Western Blotting