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Aalto Bio launches breakthrough diagnostic material for Rubella

Aalto Bio Reagents

New capsid free antigen overcomes cross-reactivity difficulties, reducing the likelihood of false positives

3 Feb 2021
Tom Casburn
Associate Editor

Product news

Aalto Bio Reagents is pleased to announce the availability of the first highly reactive recombinant Rubella Spike Ectodomain (E1-E2) antigen, code BM 6428, which has shown exceptional detection of both IgG and IgM antibodies in ELISA and bead-based immunoassays.

Purified from recombinant expression in insect cells, this high-quality antigen has guaranteed lot to lot consistency and considerably shorter lead times for large lots compared to native antigen production. Using the protein sequence from the Rubella vaccine strain HPV-77, Aalto Bio Reagents was able to produce a very reliable product, ready for use in the development of highly specific IgG and IgM assays.

Transmitted by droplets, Rubella continues to be a widespread infection, particularly in areas where vaccination rates are low. Although the disease typically presents as a mild rash in children, if contracted during pregnancy, the virus can lead to serious fetal abnormalities. Accurate detection of acute Rubella infection is therefore vital. However, reliable detection of IgM antibodies has proved challenging due to the non-specific interferences with other pathogens. It is believed this cross-reactivity is primarily linked to one of the three viral structural proteins – the capsid protein (C). By removing this protein and leaving the two major immunological targets – spike glycoproteins E1 and E2, this new antigen eliminates these potential IgM cross-reactivity issues, leading to fewer false positives.

CEO of Aalto Bio Reagents, Philip Noone, comments on how the new recombinant Rubella Spike Ectodomain (E1-E2) antigen is a strong contender for the replacement of native Rubella antigens in IVD assays – “There is a very high correlation for IgG and IgM antibody detection with our recombinant antigen versus a commercial native antigen, with our antigen showing 100% sensitivity and between 94.6% to 100% specificity in both ELISA and bead-based assays. Our capsid free antigen overcomes cross-reactivity difficulties and can clearly distinguish between positive and negative samples.”

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.ELISA

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Aalto Bio launches breakthrough diagnostic material for Rubella