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A Simple Step to Improve Your CRISPR/Cas9 Results

SelectScience

Discover how the use of high-throughput pipetting tools can facilitate this cutting-edge gene-editing tool

14 Jan 2019
Lui Terry
Administrator / Office Personnel

Editorial article

CRISPR/Cas9 is a genetic manipulation method, and the development of this revolutionary genome editing tool has enabled scientists around the world to make more accurate edits quickly and more cheaply than previously thought possible.

Like many of the molecular biology techniques, CRISPR/Cas9 is reliant on tedious 96 and 384 plate preparation, requiring consistent pipetting across microplates which can be prone to human error. To carry out effective gene editing research, therefore, it is essential that you have the right tools to hand, and Gilson has prepared a guide to help scientists maximize the levels of reproducibility in their workflow.

Cell culture is an important step in the CRISPR/Cas9 workflow and fast culture is required to maximize the probability of detecting the modification of interest. Whilst manual multichannel pipettes can be used to do this, with such large-scale workflows, manual sample preparation can often prove inefficient, time-consuming and laborious. By choosing to utilize an automated pipette such as the Gilson PLATEMASTER®, researchers have the ability to streamline their CRISPR/Cas9 studies at scale.

The Gilson PLATEMASTER

The PLATEMASTER boasts both high levels of accuracy and an intuitive operation system. The system works by reducing the number of pipetting steps necessary to fill a microplate (when compared with other regular multichannel pipettes), and it is possible to fill an entire 96-well plate in less than 10 seconds.

The PLATEMASTER is considered an ideal solution for performing both 96-well and 384-well plate-to-plate transfers or for transferring buffers or media from a reservoir. It also reduces the risk of human error, by eliminating any chance of skipping or repeating wells by accident.

The system requires zero programming and little set-up is required. This, along with its small footprint, makes it ideal to use in a laminar flow hood or cabinet and for moving around the lab as needed.

The PLATEMASTER has received positive feedback from scientists leaving reviews on the SelectScience® website. Ana Kitanovic, of IFM Therapeutics, uses the instrument for compound screening, supernatant collection and HTRF assays. She describes it as an easy-to-use instrument that can save a lot of time and says it has significantly increased screening throughput. The reviewer also highlights the "very good price," concluding:


A must have instrument for all screening labs!

Ana Kitanovica  IFM Thermapeutics


Gilson provides liquid handling solutions for each stage of the CRISPR/Cas9 workflow, download this guide to find out more.

Do you already use the PLATEMASTER? Write a review today for your chance to win an Amazon voucher worth $400 or an iPad.

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Gilson PLATEMASTER 20uL

Gilson, Inc.

PLATEMASTER® is an easy-to-use, accurate solution for high-throughput manual pipetting of 96- and 384-well microplates. Two models are available: PLATEMASTER P20 for pipetting volumes from 0.5−20 µL and PLATEMASTER P220 for pipetting volumes from 2−220 µL.  Increase the Productivity of Your Workflows The PLATEMASTER’s 96-channel design greatly reduces the number of pipetting steps necessary to fill a microplate when compared to using regular manual multichannel pipettes. When using PLATEMASTER, the time it takes to fill 96-well plates is significantly reduced to approximately 10-20 seconds or less, and 384-wells can typically be filled in less than a minute using only four pipetting steps. Increased Accuracy, Precision, and Reproducibility Using PLATEMASTER for higher throughput applications reduces the risk of human error. In a single motion, accuracy and precision levels can equal that of an 8- or 12-channel pipettes, while avoiding the risk of skipping or repeating wells. Additionally, all enzymatic reactions and cell-based applications across all wells can be started and stopped at the same time. From 0.5 µL to 220 µL pipetting volumes, expect improved well-to-well and plate-to-plate reproducibility. Versatile and Easy to Use PLATEMASTER is as intuitive as a PIPETMAN® and does not require programming, long training, or power for use. Through a variety of accessories, such as adapters for loading 384-well microplates, reagent reservoirs, or sample heater blocks, PLATEMASTER can be fully adapted to a variety of application needs. Its compact and portable design easily integrates into the lab, allowing it to fit on most benchtops, under hoods, or in cold rooms.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Scale-upScale-Up is generally defined as the process of transferring the results of the discovery phases of the drug discovery process, obtained on a laboratory scale, to the pilot plant and finally to production scale. Scale-Up uses reactors and pump systems as well as reagents, standards and buffers. Often Scale-Up is provided as a service by Contract Manufacturing Organizations (CMOs). Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Liquid HandlingLiquid handling involves the precise transfer, mixing, and dispensing of liquids in laboratory settings. It is essential for accurate and consistent results in biological, chemical, and pharmaceutical research. Automated liquid handling systems improve efficiency and reduce human error in sample preparation and analysis. Explore liquid handling tools in our peer-reviewed product directory; compare products, read customer reviews, and get pricing directly from manufacturers.CRISPRCRISPR technology enables precise editing of genes, allowing scientists to modify DNA at specific locations. This revolutionary tool is used in genetic research, drug development, and gene therapy. CRISPR has applications in agriculture, disease treatment, and creating genetically modified organisms (GMOs). Explore CRISPR solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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