A Ready-to-Use, LifeAct-Encoding mRNA for Rapid and Non-Toxic F-Actin Visualization in Cells

With their latest reagent, mRNA LifeAct-TagGFP2, ibidi has created a rapid, non-invasive and non-toxic solution for the visualization of filamentous actin (F-actin) in living and fixed eukaryotic cells.

As a key component of the cytoskeleton, F-actin orchestrates many crucial cellular processes, such as cell division and cell migration. The LifeAct peptide binds F-actin structures with a high specificity, while still keeping their full functionality. mRNA LifeAct-TagGFP2 encodes a fusion protein of LifeAct and a GFP reporter, allowing for cellular F-actin visualization with an excellent signal-to-noise ratio.

mRNA LifeAct-TagGFP2 is particularly interesting for researchers who analyze the cytoskeleton organization and actin dynamics using live cell fluorescence imaging. The fully processed, mature mRNA is optimized for stability, resulting in high levels of LifeAct peptide expression. Since the mRNA does not integrate into the host cell’s genome, no biosafety laboratory level 1 or 2 is needed.

Scientists can rapidly transfect their cells with mRNA LifeAct-TagGFP2 by using ibidi’s highly biocompatible mRNA transfection reagent, Fuse-It-mRNA easy. This new fusion reagent is ideally suited for sensitive and primary cells. As an introductory offer, a free sample of Fuse-It-mRNA easy is included with every order of the LifeAct mRNA.